Preparation and determination of egg white ovomucin.
A simple and rapid method has been developed for producing egg white ovomucin in a pure and natural form. The procedure consists of diluting the egg white sample in an appropriate buffer and applying the diluted sample to a previously equilibrated Sepharose 4B column. Since the molecular weight of o...
| Main Author: | |
|---|---|
| Other Authors: | , , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
College Station, TX :
Texas A & M University,
1970.
|
| Subjects: | |
| Online Access: | Link to ProQuest copy Link to OAKTrust copy |
MARC
| LEADER | 00000cam a2200000Ii 4500 | ||
|---|---|---|---|
| 001 | in00000174937 | ||
| 005 | 20220103135128.0 | ||
| 008 | 791204s1970 txuao bm 000 0 eng d | ||
| 035 | |a (OCoLC)5758963 | ||
| 035 | |a (OCoLC)ocm05758963 | ||
| 040 | |a TXA |b eng |c TXA |d OCL |d OCLCQ |d OCLCG |d TMV |d OCLCQ |d TXA |d OCLCO |d OCLCF |d OCLCA | ||
| 060 | 4 | |a SF 490 |b Y68p 1970 | |
| 100 | 1 | |a Young, Louis Lee. | |
| 245 | 1 | 0 | |a Preparation and determination of egg white ovomucin. |
| 264 | 1 | |a College Station, TX : |b Texas A & M University, |c 1970. | |
| 300 | |a xi, 68 leaves : |b illustrations, photographs ; |c 30 cm | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a unmediated |b n |2 rdamedia | ||
| 338 | |a volume |b nc |2 rdacarrier | ||
| 500 | |a "Major Subject: Poultry Science (Products Technology)." | ||
| 500 | |a "Submitted to the Graduate College of Texas A & M University in partial fulfillment of the requirement for the degree of Doctor of Philosophy August 1970." | ||
| 500 | |a Vita. | ||
| 502 | |b Ph. D. in Poultry Science (Products Technology) |c Texas A & M University | ||
| 504 | |a Includes bibliographical references (leaves 62-67). | ||
| 520 | 3 | |a A simple and rapid method has been developed for producing egg white ovomucin in a pure and natural form. The procedure consists of diluting the egg white sample in an appropriate buffer and applying the diluted sample to a previously equilibrated Sepharose 4B column. Since the molecular weight of ovomucin exceeds the upper exclusion limit of the Sepharose 4B, the ovomucin is eluted with the excluded volume. Ovomucin so produced contained 10.7 mg of sialic acid per 100 mg of protein, was electrophoretically nonmobile on paper electrophoresis and was precipitated by acidification and dilution with distilled water. The preparation was free of lysozyme activity but was precipitated by the addition of active lysozyme. A sample of ovomucin prepared by the gel filtration method and concentrated by ultrafiltration was periodically injected into rabbits to prepare an ovomucin antiserum. The antiserum was used to titrate the ovomucin standards. It was found that as the ovomucin content of the standards decreased, the end point in the titration decreased. The ovomucin concentration in a sample of egg white was then determined by titration against the ovomucin antiserum. An ovomucin concentration of 0.2 percent was observed which is in good agreement with published results. | |
| 650 | 2 | |a Ovomucin |x analysis. | |
| 650 | 4 | |a Major poultry science (products technology) | |
| 655 | 7 | |a Academic theses. |2 fast |0 (OCoLC)fst01726453 | |
| 655 | 7 | |a Academic theses. |2 lcgft | |
| 700 | 1 | |a Foster, B. G., |e degree committee member. | |
| 700 | 1 | |a Gardner F. A., |e degree supervisor. | |
| 700 | 1 | |a Krueger, W. F., |e degree committee member. | |
| 700 | 1 | |a Quisenberry, J. H., |e degree committee member. | |
| 700 | 1 | |a Vanderzant, C., |e degree committee member. | |
| 710 | 2 | |a Texas A & M University, |e degree granting institution. | |
| 856 | 4 | 1 | |u http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=758222821&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |z Link to ProQuest copy |t 0 |
| 856 | 4 | 1 | |u https://hdl.handle.net/1969.1/DISSERTATIONS-181583 |z Link to OAKTrust copy |t 0 |
| 948 | |h HELD BY TXA - 2 OTHER HOLDINGS | ||
| 994 | |a Z0 |b TXA | ||
| 999 | f | f | |s f202b236-e1b0-3e2c-ad7e-85359a266ef4 |i 35d7f4f6-ea64-363f-87b6-c6b3d9742025 |t 0 |
| 952 | f | f | |p noncirc |a Texas A&M University |b J.J. Pickle Campus |c High Density Repository |d Remote Storage |t 0 |e 1970 Dissertation Y73 |h Other scheme |i unmediated -- volume |m A14840941049 |
| 952 | f | f | |a Texas A&M University |b College Station |c Electronic Resources |d Available Online |t 0 |e 1970 Dissertation Y73 |h Other scheme |
| 998 | f | f | |a 1970 Dissertation Y73 |t 0 |l Remote Storage |
| 998 | f | f | |a 1970 Dissertation Y73 |t 0 |l Available Online |