Advances in the development of a somatic cell genetic system for Oryza sativa (rice) via anther culture /
Oryza sativa (Rice) was anther cultured to produce callus and subsequently plants. It was shown that these plants were pollen derived and at least a portion of them were haploid. By using a cold-shock of 13°C, about 74% of anthers with microspores at the G1 stage of the cell cycle were induced to pr...
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
[College Station, Tex.] :
Genovesi,
1978.
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| Subjects: | |
| Online Access: | Link to ProQuest copy Link to OAKTrust copy |
| Summary: | Oryza sativa (Rice) was anther cultured to produce callus and subsequently plants. It was shown that these plants were pollen derived and at least a portion of them were haploid. By using a cold-shock of 13°C, about 74% of anthers with microspores at the G1 stage of the cell cycle were induced to produce callus. An acetocarmine stain of the cold-shocked anthers showed the presence of symmetrical mitoses. Histological sections of regenerative anther callus revealed the presence of somatic embryoids. More interesting was the presence of ovary-like structures in which embryos were developing. Suspension cultures of anther derived callus were established with a goal of producing large populations of cell clumps with as few cells per clump as possible. The addition of abscisic acid, 2,4-D, and gibberellic acid to a modified Chu and Wang medium as well as the addition of adenine sulfate lead to the accomplishment of these goals. Numerous combinations of hormones and nutrients were tested to determine what parameters controlled the differentiation of aged callus into plants. As yet, this problem has not been resolved. It was possible to obtain greening of callus consistently following the culture of callus for 2 to 4 weeks at room temperature on a medium containing ABA, GA, and BAP or adenine sulfate. Chilling of the callus at 13°C for one week as well as the addition of silicate to the medium seemed to hasten and intensify greening. Further manipulation of these parameters should lead to plant regeneration. |
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| Item Description: | "Major subject: Genetics." Vita. |
| Physical Description: | x, 86 leaves : illustrations ; 28 cm |
| Bibliography: | Includes bibliographical references (leaves 81-84). |