Patch-clamp methods and protocols /

Bibliographic Details
Other Authors: Molnar, Peter, 1965-, Hickman, James J.
Format: Book
Language:English
Published: Totowa, N.J. : Humana, [2007]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 403.
Subjects:
Table of Contents:
  • Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA receptors using the whole-cell patch-clamp method
  • Memantine as an example of a fast, voltage-dependent, open channel N-methyl-D-aspartate receptor blocker
  • Methods for evaluation of positive allosteric modulators of glutamate AMPA receptors
  • Automated voltage-clamp technique
  • Flip-the-tip : automated patch clamping based on glass electrodes
  • The roboocyte : automated electrophysiology based on Xenopus oocytes
  • Infrared-guided laser stimulation as a tool for elucidating the synaptic site of expression of long-term synaptic plasticity
  • Single-cell RT-PCR, a technique to decipher the electrical, anatomical, and genetic determinants of neuronal diversity
  • Mechanosensitive ion channels investigated simultaneously by scanning probe microscopy and patch clamp
  • Synaptic connectivity in engineered neuronal networks
  • Modeling of action potential generation in NG108-15 cells
  • Whole-cell voltage clamp on skeletal muscle fibers with the silicone-clamp technique
  • Determination of channel properties at the unitary level in adult mammalian isolated cardiomyocytes
  • Electrophysiological properties of embryonic stem cells during differentiation into cardiomyocyte-like cell types
  • Hybrid neuronal network studies under dynamic clamp
  • Cardiac channelopathies studied with the dynamic action potential-clamp technique
  • Principles of single-channel kinetic analysis
  • Use of Xenopus oocytes to measure ionic selectivity of pore-forming peptides and ion channels
  • Estimation of quantal parameters with multiple-probability fluctuation analysis